Venepuncture/phlebotomy


Specifications: The station consists of an anatomical arm and all the equipment that might be
required. Assume that the anatomical arm is a patient and take blood from it.
Before starting
Introduce yourself to the patient.
Confirm his name and date of birth.
Explain the procedure and obtain his consent. For example, “I would like to take a blood sample
from you to check how your kidneys are working. This is a quick, simple, and routine procedure
which involves inserting a small needle into one of the veins on your arm. You will feel a sharp
scratch when the needle is inserted, and there may be a little bit of bleeding afterwards. Do you have
any questions?”.
Ask him from which arm he prefers to have (or normally has) blood taken.
Ask him to expose this arm.
Gather the equipment in a clean tray.
The equipment
In a clean tray, gather:
A pair of non-sterile gloves
A tourniquet
Alcohol wipes (sterets)
A 23G (blue) needle/butterfly and Vacutainer holder
The bottles appropriate for the tests that you are sending for (these vary from hospital to
hospital, but are generally yellow for biochemistry/U&Es, purple for haematology, pink for
group and save and crossmatch, blue for clotting/coagulation, grey for glucose, and black
for ESR)
Cotton wool, swab, or gauze
Tape or plaster
Make sure you have a yellow sharps box close at hand. The key to passing this station is
to be seen to be safe.
The procedure
Wash your hands (see Station 1).
Position the patient so that his arm is fully extended. Ensure that he is comfortable.
Apply the tourniquet proximal to the venepuncture site.
Select a vein by palpation: the bigger and straighter the better. The vein selected is most commonly
the median cubital vein in the antecubital fossa.
Don a pair of non-sterile gloves.
Clean the venepuncture site with an alcohol steret. Explain that this may feel a little cold.
Once the alcohol has dried off, attach the needle to the Vacutainer holder.
Tell the patient to expect a sharp scratch.
Retract the skin to stabilise the vein and insert the needle into the vein at an angle of 3045
degrees to the skin.
Clinical Skills for OSCEs
6 Station 3 Venepuncture/phlebotomy
Keeping the needle still, place a Vacutainer tube on the holder and let it fill.
Once all the necessary tubes are filled, release the tourniquet. Remember that the tubes need
to be filled in a certain order (bottles with no additives first). See the guide to Vacutainer tubes
in Station 111.
Remove the needle from the vein and apply pressure on the puncture site for at least 30
seconds (the patient may assist with this, or you may use tape or plaster).
Immediately dispose of the needle in the sharps box.
Remove and dispose of the gloves in the clinical waste bin.
Ensure that you release the tourniquet before removing the needle, and that you
immediately dispose of the needle in the sharps box.
After the procedure
Ensure that the patient is comfortable.
Thank the patient.
Label the tubes (at least: patients name, date of birth, and hospital number; date and time of
blood collection).
Fill in the blood request form (at least: patients name, date of birth, and hospital number; date
of blood collection; tests required).
Document the blood tests that have been requested in the patients notes.
Examiner’s questions
If the veins are not apparent
Lower the arm over the bedside.
Ask the patient to exercise his arm by repeatedly clenching his fist.
Gently tap the venepuncture site with two fingers.
Apply a warm compress to the venepuncture site.
Do not cause undue pain to the patient by trying over and over again (more than 23 times)
call a more experienced colleague instead.
Use femoral stab only as a last resort (usually in CPR situations).
In the event of a needlestick injury
Encourage bleeding, wash with soap and running water.
Immediately report the injury to your supervisor or the occupational health service.
If there is a significant risk of HIV, post-exposure prophylaxis should be started as soon as
possible.
Fill out an incident form.
For more information on the management of needlestick injury, refer to local or national protocols.